Background: Calcaneocuboid syndrome is a common yet underdiagnosed condition. It has been noted as a FADI scores complication of plantar flexion and inversion ankle injuries which are one of the most common athletic injuries. It is common in ballet dancers and athletes. Presentation of this condition is lateral foot pain which is exaggerated on inversion. Aim and objectives: To determine the outcomes of a single platelet-rich plasma (PRP) injection combined with rehabilitation for calcaneocuboid syndrome. Materials and methods: Six patients (3 non-sportsperson, 2 footballers, 1 basketball player) who presented with complaints of chronic lateral ankle pain were evaluated clinically and radiologically and identified to have calcaneocuboid syndrome. Ultrasound-guided PRP injection was done. Preinjection and one and three months postinjection visual analog scale (VAS) and foot and ankle disability index (FADI) scores were noted. They also underwent a rehabilitation program. Results: One patient was lost to follow-up after 1 month. Five patients showed significant improvement in VAS and FADI at 3 months. Discussion: The use of PRP has shown proven benefits in the literature. Our study combined PRP along with a rehabilitation program for the subjects. Conclusion: Single PRP injection along with a rehabilitation program has shown to be effective in the management of calcaneocuboid syndrome.